PUBLICATION

The tbx/bHLH transcription factor mga regulates gata4 and organogenesis

Authors
Rikin, A., and Evans, T.
ID
ZDB-PUB-100105-40
Date
2010
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   239(2): 535-547 (Journal)
Registered Authors
Evans, Todd
Keywords
zebrafish, cardiogenesis, gut, mesendoderm, MYC
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Apoptosis
  • Brain/embryology
  • Cloning, Molecular
  • Conserved Sequence
  • GATA Transcription Factors/metabolism*
  • Gastrointestinal Tract/embryology
  • Gene Expression Regulation, Developmental
  • Heart/embryology
  • Humans
  • Mice
  • Molecular Sequence Data
  • Organogenesis*
  • Phenotype
  • T-Box Domain Proteins/metabolism*
  • Tumor Suppressor Protein p53/metabolism
  • Zebrafish
  • Zebrafish Proteins/metabolism*
(all 19)
PubMed
20044811 Full text @ Dev. Dyn.
Abstract
The mga gene encodes a unique transcription factor containing both TBOX and bHLHzip DNA-binding domains. Here we describe the structure, expression pattern, and loss-of-function phenotype for zebrafish mga. The mga gene is conserved with mammalian homologs for both DNA-binding domains. It is expressed maternally, and subsequently in the developing brain, heart, and gut, and its depletion causes morphogenetic defects in each of these organ systems. The heart and gut phenotypes are similar to those described previously for loss of gata4, and the mga morphant shows increased levels of gata4 transcripts in lateral mesoderm. Knockdown of gata4 rescues the early heart-looping defect (but not the gut defect), indicating that mga restricts the normal levels of Gata4 required for heart tube looping, while both genes are important for gut development. Transcript profiling experiments show that mga functions early to influence key regulators of mesendoderm, including tbx6, cas, and sox17.
Genes / Markers
Marker Marker Type Name
fabp2GENEfatty acid binding protein 2, intestinal
foxa2GENEforkhead box A2
gata4GENEGATA binding protein 4
gata5GENEGATA binding protein 5
gata6GENEGATA binding protein 6
gscGENEgoosecoid
lft2GENElefty2
lhx1aGENELIM homeobox 1a
mgaaGENEMAX dimerization protein MGA a
mixl1GENEMix paired-like homeobox
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Figures
Figure Gallery (11 images) / 2
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
EGFPtwu34Tgstandard conditionsDay 5IFL
EGFPtwu34Tgstandard conditionsProtruding-mouthIFL
EGFPtwu34Tgstandard conditionsDay 5IFL
EGFPtwu34Tgstandard conditionsLong-pecIFL
EGFPtwu34Tgstandard conditionsPrim-5IFL
EGFPtwu34Tg + MO1-gata4 + MO1-mgaastandard conditionsDay 5IFL
EGFPtwu34Tg + MO1-gata4 + MO1-mgaastandard conditionsProtruding-mouthIFL
EGFPtwu34Tg + MO1-mgaastandard conditionsDay 5IFL
EGFPtwu34Tg + MO1-mgaastandard conditionsLong-pecIFL
EGFPtwu34Tg + MO1-mgaastandard conditionsDay 5IFL
1 - 10 of 109
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Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-gata4 + MO1-mgaastandard conditionsPrim-5
WT + MO1-gata4 + MO1-mgaastandard conditionsPrim-5
WT + MO1-mgaastandard conditions5-9 somites
WT + MO1-mgaastandard conditions5-9 somites
WT + MO1-mgaastandard conditions10-13 somites
WT + MO1-mgaastandard conditionsPrim-5
WT + MO1-mgaastandard conditionsPrim-5
WT + MO1-mgaastandard conditionsPrim-5
WT + MO1-mgaastandard conditionsPrim-5
WT + MO1-mgaastandard conditionsPrim-5
1 - 10 of 85
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s854TgTransgenic Insertion
    twu34TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      gata4MO1-gata4MRPHLNO
      mgaaMO1-mgaaMRPHLNO
      mgaaMO2-mgaaMRPHLNO
      mgaaMO3-mgaaMRPHLNO
      tp53MO1-tp53MRPHLNO
      1 - 5 of 5
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      Fish
      Fish
      s854Tg
      s854Tg + MO1-mgaa
      twu34Tg
      twu34Tg + MO1-gata4 + MO1-mgaa
      twu34Tg + MO1-mgaa
      WT
      WT + MO1-gata4 + MO1-mgaa
      WT + MO1-mgaa
      WT + MO1-mgaa + MO1-tp53
      WT + MO2-mgaa
      1 - 10 of 11
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      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      GFPEFGGFP
      1 - 2 of 2
      Show
      Mapping
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      Citation
      Rikin, A., and Evans, T. (2010) The tbx/bHLH transcription factor mga regulates gata4 and organogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(2):535-547.