Figure 10.

Infection-induced miR-126 regulates Cxcl12/Ccl2/Ccr2 signalling to restrict macrophage recruitment to sites of infection.

(A, B) Bacterial burden measured at 1 dpi in miR-126 and cxcl12a (A) or ccr2 (B) knockdown embryos infected with M. marinum. (C, D) Whole-body macrophage levels measured at 1 dpi in miR-126 and cxcl12a (C) or ccr2 (D) knockdown embryos infected with M. marinum. (E, F)tnfa fluorescent area at sites of infection measured at 1 dpi in miR-126 and cxcl12a (E) and ccr2 (F) knockdown embryos infected with M. marinum. (G) Measurement of macrophage levels after trunk infection with M. marinum in miR-126 knockdown and cxcl12a embryos. (H) TUNEL +ve cells counted at 3 dpi in miR-126 and cxcl12a or ccr2 knockdown embryos infected with M. marinum. Data information: each data point represents a single measurement, with the mean and SEM shown (n = 10–45) embryos per group. Bacterial burden, macrophage analysis, and granuloma tnfa graphs are representative of two experimental replicates, whereas TUNEL staining is presented as a single replicate. For macrophage time-lapse imaging, each data point represents the mean of three foci of infection from three separate embryos.