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miR-126–dependent macrophage responses to infection are not controlled by the Tsc1a/mTOR signalling axis. (A) Measurement of whole-body macrophage fluorescent area at 1 and 3 dpi in M. marinum–infected scramble control and tsc1a knockdown embryos. (B) Measurement of whole-body macrophage fluorescent area at 1 dpi in M. marinum–infected scramble control and knockdown embryos. (C) Measurement of macrophage levels after trunk infection with M. marinum in miR-126 knockdown and control embryos and treatment with either MHY1485 or rapamycin. (D) Measurement of tnfa promoter activation after trunk infection with M. marinum in knockdown embryos at 1 dpi infected with M. marinum via caudal vein injection and bacterial burden was analysed at 3 dpi. (E) Representative images of tnfa promoter-driven GFP expression at 1 dpi in knockdown embryos after trunk infection with M. marinum. M. marinum is red, and tnfa is green. Scale bar represents 100 μm. Data information: each data point represents a single measurement, with the mean and SEM shown (n = 9–56) embryos per group. Graphs are representative of two experimental replicates. For macrophage time-lapse imaging, each data point represents the mean of three foci of infection from three separate embryos.
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