Figure 6.

Decreased mTOR signalling alters cell death dynamics in mycobacterial infection.

(A) Representative images of TUNEL staining in miR-126 knockdown embryos at 3 dpi. TUNEL +ve cells are green, macrophages are magenta, and M. marinum is blue. Scale bar represents 100 μm. (B) TUNEL +ve cells in M. marinum–infected scramble control and miR-126 knockdown embryos were counted at 3 dpi. (C) Representative images of TUNEL staining in tsc1a and double knockdown embryos at 3 dpi. TUNEL +ve cells are green, and M. marinum is blue. Scale bar represents 100 μm. (D) TUNEL +ve cells in M. marinum–infected tsc1a and double knockdown embryos were counted at 3 dpi. (E) TUNEL +ve cells in rapamycin-treated, M. marinum–infected knockdown embryos were counted at 3 dpi. (F) TUNEL +ve cells in MHY1485-treated, M. marinum–infected knockdown embryos were counted at 3 dpi. Data information: each data point represents a single measurement, with the mean and SEM shown (n = 20–25 embryos per group). Graphs are representative of two experimental replicates with the exception of rapamycin/MHY1485 experiments, which were performed in a single replicate.