FIGURE

FIGURE 7

ID

ZDB-FIG-230619-7

Publication

Baldera et al., 2023 - Enhancer trap lines with GFP driven by smad6b and frizzled1 regulatory sequences for the study of epithelial morphogenesis in the developing zebrafish inner ear

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FIGURE 7

Imaging of ventral pillar formation in Et(smad6b:EGFP‐CAAX) and Et(fzd1:EGFP) ears. (A, B) Series of stills from time‐lapse movies of Et(smad6b:EGFP‐CAAX) (A, inverted greyscale) and Et(fzd1:EGFP) (B, green), showing the meeting and fusion of the ventral projection and the ventral bulge of the lateral projection to form the ventral pillar in the otic vesicle. Et(smad6b:EGFP‐CAAX) images clearly show the initial contact of cell membranes (A, arrow, 63 hpf). The Et(fzd1:EGFP) line marks the ventral projection but not the ventral bulge: GFP‐labelled and unlabelled cells can be seen meeting at the fusion plate (B, arrow, 70 hpf). Tg(xEF1A: H2B‐RFP) expression (magenta) marks all nuclei. Scale bars: 20 μm in (A) (applies to all panels); 20 μm in (B) (applies to all panels).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Anat.