ZFIN Image: Baldera et al., 2023, FIGURE 7

Image

Figure Caption

FIGURE 7

Imaging of ventral pillar formation in Et(smad6b:EGFP‐CAAX) and Et(fzd1:EGFP) ears. (A, B) Series of stills from time‐lapse movies of Et(smad6b:EGFP‐CAAX) (A, inverted greyscale) and Et(fzd1:EGFP) (B, green), showing the meeting and fusion of the ventral projection and the ventral bulge of the lateral projection to form the ventral pillar in the otic vesicle. Et(smad6b:EGFP‐CAAX) images clearly show the initial contact of cell membranes (A, arrow, 63 hpf). The Et(fzd1:EGFP) line marks the ventral projection but not the ventral bulge: GFP‐labelled and unlabelled cells can be seen meeting at the fusion plate (B, arrow, 70 hpf). Tg(xEF1A: H2B‐RFP) expression (magenta) marks all nuclei. Scale bars: 20 μm in (A) (applies to all panels); 20 μm in (B) (applies to all panels).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Anat.