Expression of the Et(fzd1:EGFP) line. (A–C') Whole mount images of Et(fzd1:EGFP) embryos taken with a Nikon CSU‐W1 spinning disk confocal microscope at 1, 2, and 3 dpf, anterior left. (a–c') Enlarged images of the otic vesicle at 1–3 dpf, corresponding to the white boxes in (A'–C′). Panels (A–C) and (a–c) are MaxIP inverted images of the EGFP expression; (A'–C′) and (a'–c') show the merged channels, with Et(fzd1:EGFP) expression in green and Tg(xEF1A:H2B‐RFP) expression in all nuclei in magenta. All are MaxIP images with the exception of (b'), which is a single z‐slice to show the GFP expression in ventral otic epithelium. (A–a') At 1 dpf, GFP expression is strongest in the retina and lens of the eye, and anterior (A, arrowhead) and posterior lateral line ganglia, with weaker expression in the midbrain and forebrain. Occasionally, some embryos also have expression in the epiphysis and heart. Expression in the otic vesicle at 1 dpf is limited to one or two cells (a arrowhead). (B–b') At 2 dpf, strong staining persists in the eye, and midbrain expression increases. New expression in the dorsal and ventral fins is strong in a subset of cells (B, arrowhead). In the otic vesicle, Et(fzd1:EGFP) expression is strongest in a ventral domain (b, b'). (C–c') At 3 dpf, expression of Et(fzd1:EGFP) is maintained in the fins and reduced in the eye and brain. Expression in the epiphysis can still be seen in some embryos (C, arrowhead). In the otic vesicle, most cells of the ventral pillar are marked by GFP expression (c, arrowhead; see also Figure 5). Scale bars: 500 μm in (A'), for (A); 500 μm in (B′), for (B′, C, C′); 50 μm in (a') for (a), in (b) for (b') and in (c) for (c').
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