Fig. 5
- ID
- ZDB-FIG-220908-27
- Publication
- Ma et al., 2022 - Ercc2/Xpd deficiency results in failure of digestive organ growth in zebrafish with elevated nucleolar stress
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Mitochondrial abnormalities, autophagy, and inflammation are highly induced (A) Transmission electron microscope images of intestinal endothelial cells (IECs) in ercc2/xpd mutants and siblings at 5 dpf. Arrows point to swollen and disarrayed mitochondria, red arrowheads point to autophagosome-like vesicles. m, mitochondrion. Scale bars, 1 μm. See also Figures S4B and S6A. (B) Immunostaining of the mitochondrial marker MT-CO1 in IECs in ercc2/xpd mutants and siblings at 5 dpf. Areas of dashed boxes are magnified. Scale bars, 20 μm. (C) Immunostaining of the autophagosomal marker LC3BII in IECs in ercc2/xpd mutants and siblings at 5 dpf. Areas of dashed boxes are magnified. Scale bars, 10 μm. (D) Transcriptomic analysis revealed increased expression of genes related to inflammation in ercc2/xpd mutants compared to siblings at 5 dpf. (E) qPCR verification of inflammation-related gene expression in ercc2/xpd mutants and siblings at 5 and 7 dpf. Data are presented as mean ± SD from three independent biological repeats. Student’s t test, ∗, p < 0.05, ∗∗, p < 0.01, NS, non-significant. See also Figure S6. |
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| Stage Range: | Day 5 to Days 7-13 |
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| Stage Range: | Day 5 to Days 7-13 |