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Fig. 5
Mitochondrial abnormalities, autophagy, and inflammation are highly induced
(A) Transmission electron microscope images of intestinal endothelial cells (IECs) in ercc2/xpd mutants and siblings at 5 dpf. Arrows point to swollen and disarrayed mitochondria, red arrowheads point to autophagosome-like vesicles. m, mitochondrion. Scale bars, 1 μm. See also Figures S4B and S6A.
(B) Immunostaining of the mitochondrial marker MT-CO1 in IECs in ercc2/xpd mutants and siblings at 5 dpf. Areas of dashed boxes are magnified. Scale bars, 20 μm.
(C) Immunostaining of the autophagosomal marker LC3BII in IECs in ercc2/xpd mutants and siblings at 5 dpf. Areas of dashed boxes are magnified. Scale bars, 10 μm.
(D) Transcriptomic analysis revealed increased expression of genes related to inflammation in ercc2/xpd mutants compared to siblings at 5 dpf.
(E) qPCR verification of inflammation-related gene expression in ercc2/xpd mutants and siblings at 5 and 7 dpf. Data are presented as mean ± SD from three independent biological repeats. Student’s t test, ∗, p < 0.05, ∗∗, p < 0.01, NS, non-significant. See also Figure S6.