FIGURE

Fig. 8

ID

ZDB-FIG-220202-12

Publication

Manchanda et al., 2021 - Truncation of the otoferlin transmembrane domain alters the development of hair cells and reduces membrane docking

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Fig. 8

The otofB mutation results in abnormal glutamate decarboxylase and GABA receptor subunit transcripts levels. (A–C) Quantification of mRNA by qRT-PCR for GABA receptor subunits and GAD transcripts in wild-type and otofB larvae at 72, 96, and 120 hpf. N = 4 for both WT and mutant, with each N as a composite of 20–30 larvae. (D) Quantification of mRNA by qRT-PCR for GABA receptor subunit and GAD transcripts in wild-type and otofB adult zebrafish brain (t test, p < 0.001). N = 4 for both WT and mutant, with each N as a composite of 20–30 larvae. The data presented are representative of three experimental replicates for each time point. Error bars are SEM.

Expression Data

Genes:	gabbr1a gabbr1b gabbr2 gabra1 gabra2b gabra6a gabra6b gabrb2a gabrb4 gabrd gabrg2 gad1a gad1b gad2
Fish:	AB otofb^{sa16297/sa16297} (AB)
Anatomical Term:	whole organism
Stage Range:	Protruding-mouth to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	otofb^{sa16297/sa16297} (AB)
Observed In:	whole organism
Stage Range:	Protruding-mouth to Adult

Phenotype Detail

Acknowledgments

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