Figure 12

The ALT is a preserved structure between distant teleost species. Representative acquisitions of anti-ZAP70 (brown/red hot) labeled gills section, from different fish species, displaying the side of a gill arch, at the base of filaments. (A, B) ALT of the European carp (Cyprinus carpio); Cyprinid – Paraffin section. (C) ALT of the Atlantic salmon (Salmo salar L.); Salmonid – cryosections stained with phalloidin (green) and DAPI (blue). (D) ALT of the Atlantic mackerel (Scomber scombrus); Scombridae, Percomorph – Paraffin section. (E) ALT of the Northern red snapper (Lutjanus campechanus); Lutjanidae, Percomorph – Paraffin section. (F) ALT of the Pacific barracuda (Sphyraena argentea); Sphyraenidae, Percomorph – Paraffin section. (G) ALT of the Medaka (Oryzias latipes); Adrianichthyidae, Percomorph - cryosections stained with phalloidin (green) and DAPI (blue). These images are maximum intensity projections: 2 µm (G) and 5 µm (C). Annotations: ALT, Amphibranchial Lymphoid Tissue; Bc, Branchial cavity; Bm, Basement membrane; Ea, Efferent artery; Ep, Epithelial cells; F, Filament; Ga, Gill arch; Gr, Gill raker; La, Lamella; S, Septum and Sm, Smooth muscles. Scale bars: 50 µm (B, G) and 70 μm (A, C).