FIGURE

Fig. 3

ID

ZDB-FIG-210814-27

Publication

Cacialli et al., 2021 - A connexin/ifi30 pathway bridges HSCs with their niche to dampen oxidative stress

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Fig. 3

<italic>Ifi30</italic> overexpression increases the number of HSPCs in the CHT and rescues the loss of HSPCs after ROS challenge.

a Experimental outline to generate double-transgenic kdrl:Gal4;UAS:ifi30. b WISH for ifi30 at 24 hpf in kdrl:Gal4⁺ embryos that were either UAS:ifi30 negative (upper) or positive (lower). c WISH for cmyb at 4 dpf in kdrl:Gal4⁺ embryos that were either UAS:ifi30 negative (upper) or positive (lower). d WISH against cmyb at 48 hpf in kdrl:Gal4⁺ embryos that were either UAS:ifi30^- (upper) or UAS:ifi30⁺ (lower), NT and H₂O₂-treated. e Quantification of cmyb-expressing cells. Statistical analysis: one-way ANOVA with Tukey–Kramer post hoc tests, adjusted for multiple comparisons, **P = 0.013; ****P < 0.0001. Center values of all statistical analyses denote the mean, and error values denote s.e.m. Scale bars: 200 μm (b, c), 100 μm (d).

Expression Data

Genes:	ifi30a myb
Fish:	bw9Tg/bw9Tg ifi30a^{sa19758/sa19758} AB + MO1-ifi30a bw9Tg/bw9Tg; ug107Tg/ug107Tg
Condition:	chemical treatment by environment: hydrogen peroxide
Knockdown Reagent:	MO1-ifi30a
Anatomical Terms:	caudal hematopoietic tissue hematopoietic multipotent progenitor cell posterior cardinal vein endothelial cell
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bw9Tg/bw9Tg ifi30a^{sa19758/sa19758} AB + MO1-ifi30a bw9Tg/bw9Tg; ug107Tg/ug107Tg
Condition:	chemical treatment by environment: hydrogen peroxide
Knockdown Reagent:	MO1-ifi30a
Observed In:	caudal hematopoietic tissue hematopoietic multipotent progenitor cell posterior cardinal vein endothelial cell
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

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