FIGURE

Figure 2

ID

ZDB-FIG-201130-74

Publication

Lu et al., 2020 - Generation and Application of the Zebrafish heg1 Mutant as a Cardiovascular Disease Model

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Figure 2

The generation of heg1 deficient zebrafish using CRISPR/Cas9 technology. (A) Schematic view of zebrafish heg1 genomic and protein domains. The gRNA sequence for the exon 1 targeting site was labelled by a red line. The CRISPR/Cas9-induced mutation (25-base deletion) in heg1 exon1 and a truncated 12 amino acids at the N-terminus were shown. (B) Sanger sequencing confirmed the 25-nt deletion mutation. (C) qRT-PCR confirmation that heg1 expression was significantly decreased in heg1^∆²⁵ embryos, n = 30 embryos per group. Data are represented as mean ± SE. *** p < 0.001.

Expression Data

Gene:	heg1
Fish:	heg1^nwc1/nwc1
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	heg1^nwc1/nwc1
Observed In:	whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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