IMAGE

Figure 2

ID

ZDB-IMAGE-201130-74

Genes

heg1

Source

Figures for Lu et al., 2020

Image

Figure Caption

Figure 2

The generation of heg1 deficient zebrafish using CRISPR/Cas9 technology. (A) Schematic view of zebrafish heg1 genomic and protein domains. The gRNA sequence for the exon 1 targeting site was labelled by a red line. The CRISPR/Cas9-induced mutation (25-base deletion) in heg1 exon1 and a truncated 12 amino acids at the N-terminus were shown. (B) Sanger sequencing confirmed the 25-nt deletion mutation. (C) qRT-PCR confirmation that heg1 expression was significantly decreased in heg1^∆²⁵ embryos, n = 30 embryos per group. Data are represented as mean ± SE. *** p < 0.001.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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