FIGURE

Fig. 3

ID

ZDB-FIG-170822-3

Publication

Ke et al., 2017 - Gβ1 is required for neutrophil migration in zebrafish

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Fig. 3

Gβ1, but not Gβ4, is required for wound-induced neutrophil migration. Epifluorescence time-lapse experiments tracking neutrophil migration for 2 h following wounding of the indicated 72 hpf-Tg(mpx:GFP) embryos. (A-C′′) Overlay of epifluorescence and bright-field images of the whole embryo (A-C) and the tail region, immediately after wounding (0 h, A'-C') and 2 h after wounding (2, A′′-C′′). Yellow lines: areas of wounding. (D) Number of neutrophils that reach the wound sites within 2 h of wounding (the number of embryos analyzed is indicated).

Expression Data

Gene:	GFP
Fish:	i114Tg i114Tg + MO1-gnb1a + MO2-gnb1b + MO4-tp53 i114Tg + MO1-gnb4a + MO1-gnb4b + MO4-tp53
Condition:	amputation: caudal fin
Knockdown Reagents:	MO1-gnb1a MO1-gnb4a MO1-gnb4b MO2-gnb1b MO4-tp53
Anatomical Term:	neutrophil
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	i114Tg + MO1-gnb1a + MO2-gnb1b + MO4-tp53 i114Tg + MO1-gnb4a + MO1-gnb4b + MO4-tp53 i114Tg + MO2-gnb1a + MO3-gnb1b + MO4-tp53
Condition:	amputation: caudal fin
Knockdown Reagents:	MO1-gnb1a MO1-gnb4a MO1-gnb4b MO2-gnb1a MO2-gnb1b MO3-gnb1b MO4-tp53 (all 7)
Observed In:	neutrophil chemotaxis neutrophil migration
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 428(1), Ke, W., Ye, D., Mersch, K., Xu, H., Chen, S., Lin, F., Gβ1 is required for neutrophil migration in zebrafish, 135-147, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.