FIGURE

Fig. 4

ID

ZDB-FIG-170822-4

Publication

Ke et al., 2017 - Gβ1 is required for neutrophil migration in zebrafish

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Fig. 4

Gβ1 function in neutrophils is required for wound-induced neutrophil migration. (A) Schematic diagram depicting transplantation procedure. Cells from 72 hpf-Tg(mpx:GFP) embryos (donor) were transplanted into wildtype (WT) embryos (host) at the sphere stage. (B-C) Snapshots from epifluorescence and bright-field time-lapse movie (Supplementary Movie 3), showing positions of neutrophils and wound sites immediately (0 h, B, C) and 2 h (2 h, B′, C′) after wounding. (B-B′) gnb1ab MO1-treated host embryos transplanted with cells from control Tg(mpx:GFP) embryo, with wound site (yellow arrows) and nearest GFP-expressing neutrophil (red arrows) indicated. In 6 out of 7 host embryos, transplanted mpx-GFP expressing neutrophils migrated to the wound. (C-C′) Control host embryos transplanted with cells from gnb1a/b MO1-injected Tg(mpx:GFP) embryo, with wound site (yellow line) and nearest GFP-expressing neutrophil (red arrows) indicated. In 6 host embryos, none of the transplanted mpx-GFP expressing neutrophils migrated to the wound. Drawing at top shows wound position and area imaged (green box).

Expression Data

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 428(1), Ke, W., Ye, D., Mersch, K., Xu, H., Chen, S., Lin, F., Gβ1 is required for neutrophil migration in zebrafish, 135-147, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.