FIGURE

Fig. 5

ID

ZDB-FIG-161027-13

Publication

Cox et al., 2016 - Selenoprotein H is an essential regulator of redox homeostasis that cooperates with p53 in development and tumorigenesis

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Fig. 5

seph mutants are sensitive to DNA damage. (A) Immunoblot analysis of p53 expression in WT and seph mutant larvae at 5 dpf. (B) Survival of WT and seph mutant larvae 2 dpe to UV irradiation (200 J/m², 7 dpf). n = 7. ****P < 0.0001. (C) Survival of WT and seph mutant larvae 2 dpe to camptothecin (1 µM). n = 10. ****P < 0.0001. (D) Morphological and histological analysis of WT, seph^-/- and tp53^-/-;seph^-/- mutant larvae at 5 dpf. The yellow dashed region represents the liver. The region of histology showing the larval liver is shown in the zoomed images. [Scale bars: 200 µm (brightfield; BF) and 50 µm (histology; H+E).] (E) Quantitative analysis of liver, pancreas, and gut area in WT, seph^-/-, and tp53^-/-;seph^-/- mutant larvae at 5 dpf. n > 8. *P < 0.05; ****P < 0.0001.

Expression Data

Gene:	tp53
Antibody:	Ab11-tp53
Fish:	AB selenoh^{hi2737Tg/hi2737Tg}
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	selenoh^{hi2737Tg/hi2737Tg} tp53^zdf1/zdf1; selenoh^{hi2737Tg/hi2737Tg}
Conditions:	chemical treatment: camptothecin ultraviolet light
Observed In:	DNA damage response eye gut hepatocyte hepatocyte apoptotic process liver pancreas whole organism
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

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