FIGURE

Fig. S10

ID

ZDB-FIG-140910-7

Publication

Pfefferli et al., 2014 - Specific NuRD components are required for fin regeneration in zebrafish

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Fig. S10

Hdac1 inhibition and chd4a knockdown do not result in the activation of the apoptosis marker caspase-3.

(A-F) Longitudinal sections of fin regenerates stained with active caspase-3 antibody (green) and with DAPI (blue). Only a few cells positive for active caspase-3 are present in control fins (A,C). Fins treated with MGCD0103 (B) or injected with chd4a MO (D) do not display any increase of apoptosis at 4 dpa. (E-F) As a positive control, the activation of caspase-3 was assessed in fin regenerates at 1 dpa treated with the chemical inhibitor of Igf1 receptor NVP_AEW541. Inhibition of IGF signaling blocks fin regeneration and results in the increase of cell apoptosis in the wound epidermis (Chablais et al., 2010). Dashed lines indicate the amputation plane. Scale bars: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB AB + MO1-chd4a
Conditions:	amputation: fin amputation: fin, chemical treatment: pharmaceutical
Knockdown Reagent:	MO1-chd4a
Observed In:	apoptotic process
Stage:	Adult

Phenotype Detail

Acknowledgments

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