FIGURE

Fig. 6

ID

ZDB-FIG-140902-24

Publication

Pfefferli et al., 2014 - Specific NuRD components are required for fin regeneration in zebrafish

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Fig. 6

Hdac1 inhibition and morpholino-mediated knockdown of chd4a, mta2, and the two rbb4 orthologs cause abnormal expression of Actinodin 1. (A-B) Longitudinal sections of fin regenerates at 4 dpa treated with DMSO (A) or MGCD0103 (B) and stained with Tenascin C antibody (red) and DAPI (blue). Mesenchymal remodeling was not altered in MGCD0103-treated fins. (C-H) Longitudinal sections of fin regenerates at 4 dpa treated with DMSO (C) or MGCD0103 (D), or injected with control (E), chd4a (F), mta2 (G), or rbb4 + rbb4l (H) MOs stained with Actinodin 1 antibody (green) and DAPI (blue). Depletion of the NuRD components hdac1, chd4a, mta2, or rbb4/rbb4l resulted in a disorganized expression pattern of Actinodin 1. Dashed lines indicate the amputation plane. Scale bars: 100 μm.

Expression Data

Gene:	and1
Antibody:	Ab2-and1
Fish:	AB AB + MO1-chd4a AB + MO2-mta2 AB + MO1-rbb4l + MO1-rbbp4
Conditions:	amputation: fin amputation: fin, chemical treatment: pharmaceutical
Knockdown Reagents:	MO1-chd4a MO1-rbb4l MO1-rbbp4 MO2-mta2
Anatomical Term:	regenerating fin actinotrichium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB AB + MO1-chd4a AB + MO2-mta2 AB + MO1-rbb4l + MO1-rbbp4
Conditions:	amputation: fin amputation: fin, chemical treatment: pharmaceutical
Knockdown Reagents:	MO1-chd4a MO1-rbb4l MO1-rbbp4 MO2-mta2
Observed In:	regenerating fin caudal fin actinotrichium
Stage:	Adult

Phenotype Detail

Acknowledgments

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