Fig. S3

Reptin genetically interacts with the cilia biogenesis gene ift172, but is dispensable for cilia biogenesis. (A) Phenotypes of reptin^hi2394; ift172^hi2211 double mutants (double) and single mutants of either genes at 4 dpf. WT, wild-type sibling. (B) Percentage of embryos showing body curvature (Left) and kidney cysts (Right). A total of 82.2% embryos show kidney cyst formation and 84.6% embryos show body curvature in the reptin MO and ift172 MO coinjection group (rep+ift), compared with 4.7% cyst formation and 5.9% body curvature in the reptin MO and control MO coinjection group (rep) or 1.5% cyst formation and body curvature in the ift172 MO and control MO coinjection group (ift). Data are represented as mean + SD from three replicates. **P < 0.01. (C) Immunostaining showing cilia (stained with antiacetylated tubulin in green) in reptinhi2394 mutants in the middle region of the pronephric duct (stained with anti-Cdh17, in red, lined with dotted lines). WT, wild type; rep, reptin^hi2394. (Scale bar, 20 μm.)