ZFIN Image: Zhao et al., 2013, Fig. S3

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Figure Caption

Fig. S3

Reptin genetically interacts with the cilia biogenesis gene ift172, but is dispensable for cilia biogenesis. (A) Phenotypes of reptin^hi2394; ift172^hi2211 double mutants (double) and single mutants of either genes at 4 dpf. WT, wild-type sibling. (B) Percentage of embryos showing body curvature (Left) and kidney cysts (Right). A total of 82.2% embryos show kidney cyst formation and 84.6% embryos show body curvature in the reptin MO and ift172 MO coinjection group (rep+ift), compared with 4.7% cyst formation and 5.9% body curvature in the reptin MO and control MO coinjection group (rep) or 1.5% cyst formation and body curvature in the ift172 MO and control MO coinjection group (ift). Data are represented as mean + SD from three replicates. **P < 0.01. (C) Immunostaining showing cilia (stained with antiacetylated tubulin in green) in reptinhi2394 mutants in the middle region of the pronephric duct (stained with anti-Cdh17, in red, lined with dotted lines). WT, wild type; rep, reptin^hi2394. (Scale bar, 20 μm.)

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA