Fig. 2

Reptin is essential for cilia motility. (A and B) Kymographs showing the rhythmic beating of a multicilia bundle in the posterior pronephric duct in a wild-type (WT) embryo and paralyzed cilia in a reptin^hi2394 mutant (rep), or a slower beating cilium in a seahorse^hi3308 mutant (sea) at 3 dpf. (C) Cilia beating frequency in wild-type (black bar) and seahorse^hi3308 (white bar) pronephros at 3 dpf. Data are represented as mean + SD, from eight replicates. *P < 0.05. (D) Cilia beating frequency in the olfactory placode of wild-type (black bar, WT) and seahorse^hi3308 mutant (white bar, sea) embryos at 3 dpf. Data are represented as mean + SD, from five replicates. *P < 0.05. (E) Electron micrographs of cross-sections of cilia in WT and reptin^hi2394 mutants (rep) at 5 dpf. Outer and inner dynein arms are pointed out by white and black arrowheads, respectively. (F) Number of outer and inner dynein arms in WT and reptin^hi2394 mutants (rep) per section at 5 dpf. Data are represented as mean + SD. **P < 0.01. (G) Expression levels of multiple factors involved in dynein arm formation as shown by quantitative PCR (qPCR). Y axis shows the fold changes in reptin^hi2394 mutants. Data are represented as mean + SD, from three replicates. *P < 0.05, **P < 0.01. NS, nonsignificant.