FIGURE

Fig. 6

ID

ZDB-FIG-130726-40

Publication

Melvin et al., 2013 - A morpholino-based screen to identify novel genes involved in craniofacial morphogenesis

Other Figures

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Fig. 6

Progressive and dose-dependent loss of the neural crest–derived cranial skeleton in Class III morphants. A, D–E′: vgll4l morphants. F–H′: tshz2 morphants. I–K′: pkdcc morphants. A, F, I, Lateral view of 5dpf zebrafish after injection with gene-specific Morpholino. B and C, Ventral view of uninjected larva at 5dpf. D, E, G, H, J, and K, Ventral view of mild morphant phenotypes at 5dpf. D′, E′, G′, H′, J′, and K′, Ventral view of severe morphant phenotypes. Bottom: Magnified images of craniofacial skeletons. Dose and Morpholino are indicated in the figure. The arrowheads in G′, J′, and K′ indicate the remnants of the viscerocranial cartilages. Asterisk indicates fused trabecula and reduction of the ethmoid plate. Scale bars = 500 μm in A, B, and C. Abbreviations as in Figure 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-pkdccb WT + MO1-tshz2 WT + MO1-vgll4l WT + MO2-pkdccb WT + MO2-tshz2 WT + MO2-vgll4l
Knockdown Reagents:	MO1-pkdccb MO1-tshz2 MO1-vgll4l MO2-pkdccb MO2-tshz2 MO2-vgll4l
Observed In:	ceratobranchial cartilage ceratohyal cartilage chondrocranium cartilage cranial cartilage ethmoid cartilage eye head anterior side hyosymplectic cartilage Meckel's cartilage neurocranial trabecula palatoquadrate cartilage ventral mandibular arch yolk
Stage:	Day 5

Phenotype Detail

Acknowledgments

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