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Fig. 6 Progressive and dose-dependent loss of the neural crest–derived cranial skeleton in Class III morphants. A, D–E′: vgll4l morphants. F–H′: tshz2 morphants. I–K′: pkdcc morphants. A, F, I, Lateral view of 5dpf zebrafish after injection with gene-specific Morpholino. B and C, Ventral view of uninjected larva at 5dpf. D, E, G, H, J, and K, Ventral view of mild morphant phenotypes at 5dpf. D′, E′, G′, H′, J′, and K′, Ventral view of severe morphant phenotypes. Bottom: Magnified images of craniofacial skeletons. Dose and Morpholino are indicated in the figure. The arrowheads in G′, J′, and K′ indicate the remnants of the viscerocranial cartilages. Asterisk indicates fused trabecula and reduction of the ethmoid plate. Scale bars = 500 μm in A, B, and C. Abbreviations as in Figure 1.