FIGURE

Fig. 1

ID
ZDB-FIG-130624-15
Publication
Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish
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Fig. 1

Endocardial and cardiac valve defects were found in scote382 mutant embryos. (A,B) Brightfield views of live wild-type (A) and scote382 mutant (B) embryos at 48hpf. Note pericardial edema and heart abnormality (black arrowhead), as well as abnormal vessels in the caudal trunk (red arrowhead). (C,D) Confocal images of Tg(myl7:eGFP) transgenic wild-type (C) and scote382 mutant (D) hearts at 48hpf. Note lack of constricted AVC and dilated heart in scote382 mutant (arrowhead). (E,F) Hematoxylin-eosin staining of wild-type (E) and scote382 mutant (F) hearts at 48hpf on transverse JB4 sections. Note lack of AVC constriction (arrowhead) in scote382 mutant heart. (G,H) Valve leaflets (arrowhead) were labeled and detected by the Tg(kdrl:eGFP) transgene in wild-type (G), but not in scote382 mutant (H) embryos at 6dpf on vibrotome sections across the heart. (I–N) Endocardial markers fli1 (I,J) and tie1 (K,L) were expressed in wild-type (I,K) and scote382 mutant (J,L) embryos; and Tg(kdrl:eGFP) labeled the endocardium in wild type (M) and scote382 mutant (N) embryos at 48hpf. Note that endocardial genes (fli1, tie1 and flk1) were reduced in the atrium of scote382 mutants (J,L,N). Arrows point to the AVC. a, atrium; v, ventricle.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 6

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Long-pec to Day 6

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.