FIGURE

Fig. 6

ID

ZDB-FIG-130624-20

Publication

Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Cardiac valve genes were abnormally expressed in the AVC of sco^te382 mutant hearts. (A-R) Wild-type (A-C,G-I, M-N,Q) and sco^te382 (D-F,J–L,O-P,R) embryos were analyzed by in situ hybridization with cardiac valve markers notch1b (A-F), bmp4 (G–L), dll4 (M-P) and spp1 (Q–R) at 48hpf (A,D,G,J), 60hpf (B,E,H,K,M,O), and 72hpf (C,F,I,L,N,P–R). Note that endocardial notch1b and dll4 were ectopically expressed in the ventricle of sco^te382 mutants but myocardial bmp4 was not affected in sco^te382 mutants from 48 to 72hpf. spp1, an endocardial–mesenchymal transition marker gene, was not expressed in the AVC of sco^te382 mutants (red arrowhead in R). The atrium and ventricle are demarcated with red arrowheads. Black arrowheads (Q,R) point to the outflow tract. a, atrium; v, ventricle; wt, wild type. Dotted lines outline the heart.

Expression Data

Genes:	bmp4 dll4 notch1b spp1
Fish:	WT fbn2b^{te382a/te382a}; pku300^{te382b/te382b}
Anatomical Terms:	atrioventricular canal atrioventricular canal endocardial cushion atrioventricular canal endocardium bulbus arteriosus bulbus arteriosus myocardium sinus venosus myocardium ventricular endocardium
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fbn2b^{te382a/te382a}; pku300^{te382b/te382b}
Observed In:	endocardial cushion to mesenchymal transition involved in heart valve formation Notch signaling involved in heart development
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.