FIGURE

Fig. 3

ID
ZDB-FIG-130624-17
Publication
Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish
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Fig. 3

Mutations of both pku300 and fbn2b contributed to scote382 mutant phenotypes. The endocardium in the atrium, ventricle and CCV were labeled by the Tg(kdrl:EGFP) transgene. The endocardium was projected by red pseudocolor with Imaris software. (A-C) 2341 offspring from heterozygous scote382 mutant crosses produced 25.5% mutants that had defects in the atrial endocardium and CCV (B), 6.7% mutants that had only reduced atrial endocardium (C), and 67.8% siblings that were phenotypically normal (A), suggesting more than one gene mutated in the scote382 locus. Sequencing confirmed that the mutants (group II) with defects in the heart (endocardium and CCV) and tail (caudal vein) (supplementary material Fig. S3) were fbn2b-/- and pku300-/- (B), and the mutants (group I) with defects only in the heart (endocardium) were fbn2b+/- and pku300+/- (C). (D–F) 297 offspring from heterozygous scopku300 mutant crosses produced 25.5% mutants with defects in the atrial endocardium and CCV (E) and 74.6% normal siblings (D), which was supported by morpholino knockdown of pku300 with 9.6ng pku300MO (F). (G–I) A fbn2bMO (1.6ng) morphant showed reduced atrial endocardium but had normal CCV (I), compared with the control (H) and a pku300 morphant (G). a, atrium; v, ventricle. Scale bars: 50μm.

Expression Data
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.