FIGURE

Fig. 8

ID

ZDB-FIG-130624-22

Publication

Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish

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Fig. 8

Endocardial cell adhesion and tight junctions were ectopically expressed in the AVC of sco^te382 mutant hearts. (A–F) Endocardial cell adhesion gene cdh5/ve-cadherin was analyzed by in situ hybridization in wild-type embryos at 48hpf (A), 60hpf (B) and 72hpf (C); and sco^te382 mutant embryos at 48hpf (D), 60hpf (E) and 72hpf (F). Note gradual restriction of cdh5 in the AVC in wild-type embryos and ectopic expression of cdh5 in the ventricle of sco^te382 mutant embryos. Red arrowheads point to the AVC. (G–L) Wild-type and sco^te382 mutant Tg(kdrl:EGFP) transgenic embryos on vibratome sections were subjected to immunostaining with anti-ZO1 antibody. Note that ZO1 was expressed in the AVC of wild-type hearts at 48hpf (G) and downregulated at 60 (I) and 72hpf (K), but remained in the AVC of sco^te382 mutant hearts at 48 (H), 60 (J) and 72hpf (L). a, atrium; v, ventricle; arrowhead points to the AVC. Dotted lines outline the heart.

Expression Data

Genes:	cdh5 GFP
Antibody:	Ab1-tjp1
Fish:	WT la116Tg fbn2b^te382a/+; pku300^te382b/+; la116Tg/+ fbn2b^{te382a/te382a}; pku300^{te382b/te382b} fbn2b^{te382a/te382a}; pku300^{te382b/te382b}; la116Tg
Anatomical Terms:	atrial endocardium atrioventricular canal endocardium atrioventricular canal endocardium bicellular tight junction endocardium ventricular endocardium
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fbn2b^te382a/+; pku300^te382b/+ fbn2b^{te382a/te382a}; pku300^{te382b/te382b}
Observed In:	atrioventricular canal endocardium cell adhesion involved in heart morphogenesis
Stage Range:	Pec-fin to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.