FIGURE

Fig. 5

ID

ZDB-FIG-130123-24

Publication

Bosco et al., 2013 - Development of hypothalamic serotoninergic neurons requires Fgf signalling via the ETS-domain transcription factor Etv5b

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Fig. 5

Development of hypothalamic 5-HT neurons depends on Fgf signalling. (A,B) Micrograph showing DMSO control and SU5402-exposed zebrafish embryos processed for etv5b ISH. Following SU5402 treatment, no etv5b transcripts were detected in the hypothalamus (h). Lateral view, anterior left. (C-F) Confocal maximum intensity projections showing control and SU5402-exposed embryos processed for 5-HT immunohistochemistry. The intermediate/posterior (i./p.) clusters of the hypothalamus (C,D) and the 5-HT population of the anterior raphe (r.) (E,F) are indicated. Ventral views, anterior left. (G) The number of 5-HT cells after SU5402 exposure during the indicated time intervals in clusters i./p. and r. at 72 hpf expressed as percentage of control (dashed line at 100%). ^*Pd0.05; n.s., not significant. (H-O) nkx2.1a expression in DMSO controls and SU5402-exposed embryos. Lateral (H-K) and ventral (L-O) views, anterior left. Scale bars: 50 μm.

Expression Data

Genes:	etv5b nkx2.4b
Antibody:	Ab1-serotonin
Fish:	WT
Condition:	chemical treatment: SU5402
Anatomical Terms:	caudal tuberculum epiphysis forebrain ventral region hypothalamus midbrain hindbrain boundary optic stalk raphe nucleus telencephalon
Stage Range:	Prim-25 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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