FIGURE

Fig. 2

ID

ZDB-FIG-130123-21

Publication

Bosco et al., 2013 - Development of hypothalamic serotoninergic neurons requires Fgf signalling via the ETS-domain transcription factor Etv5b

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Fig. 2

etv5b-deficient embryos exhibit fewer 5-HT neurons in the hypothalamus. (A-J) Confocal maximum intensity projections showing controls and etv5b morphants processed for 5-HT immunohistochemistry. The intermediate/posterior (i./p.) clusters of the hypothalamus (A,B,E-G) and the 5-HT population of the anterior raphe (r.) are indicated (C,D,H-J). Ventral views, anterior left. (K-T) nkx2.1a expression in the basal forebrain. Lateral (K,L,O-Q) and ventral (M,N,R-T) views, anterior left. (U,V) The number of 5-HT cells at 72 hpf in controls and etv5b ATG (0.075 mM, U) or splice (0.15 mM, V) morphants expressed as percentage of control. n, total number of embryos analysed for each experiment. ^*Pd0.05; n.s., not significant. Scale bars: 50 μm.

Expression Data

Gene:	nkx2.4b
Antibody:	Ab1-serotonin
Fish:	WT WT + MO2-etv5b WT + MO3-etv5b
Knockdown Reagents:	MO2-etv5b MO3-etv5b
Anatomical Terms:	forebrain ventral region hypothalamus raphe nucleus
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-etv5b WT + MO3-etv5b
Knockdown Reagents:	MO2-etv5b MO3-etv5b
Observed In:	hypothalamus serotonin secreting cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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