FIGURE

Fig. 7

ID

ZDB-FIG-130123-27

Publication

Bosco et al., 2013 - Development of hypothalamic serotoninergic neurons requires Fgf signalling via the ETS-domain transcription factor Etv5b

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Fig. 7

Etv5b regulates cell cycle progression in hypothalamic progenitors. (A) The procedure for analysis of cell cycle kinetics of hypothalamic progenitors. (B,C) The number of BrdU, ph-H3, Fucci total (mAG:hGem and mKO2:zCdt1), mAG:hGem or mKO2:zCdt1 immunoreactive cells at the caudal levels of the hypothalamus at 36 hpf expressed as percentage of control. ^*Pd0.05. These data suggest that hypothalamic progenitors in etv5b morphants display a longer S phase or are blocked in S phase at 36 hpf. (D-K) Confocal optical sections of coronal cryosections from etv5b morphants or uninjected controls (wild type, D-G; Fucci transgenics, H-K). Wild-type morphants and controls were exposed to a single pulse of BrdU at 36 hpf and processed for BrdU and ph-H3 double immunohistochemistry, whereas Fucci transgenic morphants and controls were processed for mAG:hGem and mKO2:zCdt1 double immunohistochemistry. Sections at the caudal level of the hypothalamus (as indicated in inset in D) are shown. Dotted lines indicate the border of caudal hypothalamus within which cells were counted. Examples of immunoreactive cells are indicated (arrowheads). Scale bar: 50 μm.

Expression Data

Antibody:	Ab4-h3
Fish:	WT WT + MO3-etv5b
Knockdown Reagent:	MO3-etv5b
Anatomical Term:	hypothalamus
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-etv5b
Knockdown Reagent:	MO3-etv5b
Observed In:	hypothalamus mitotic S phase
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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