FIGURE

Fig. 8

ID

ZDB-FIG-120315-80

Publication

Shen et al., 2012 - The cytokine macrophage migration inhibitory factor (MIF) acts as a neurotrophin in the developing inner ear of the zebrafish, Danio rerio

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Fig. 8

Statoacoustic ganglion in the mif, iclp morphants and 4-IPP treated embryos. (A–F) Whole mount in situ hybridization of embryos with an nkx5.1 probe, showing the otic vesicle and SAG at 32 hpf. (A) control embryo; (B) mif morphant; (C) mif morphant with RNA; (D) iclp1 morphant; (E, F) 4-IPP treated (E: 30 μM, F: 40 μM) embryos. (G–K) zn-5 staining in 48-hpf embryos. (G) control; (H) mif morphant; (I) mif morphant with capped mif RNAs; (J) iclp morphant; (K) iclp morphant with capped iclp RNAs. The white line outlines the SAGs. Note other cranial ganglia were also smaller in the mif morphants than the control. gAD: anterodorsal LL ganglia. Scale bar: 25 μm. (L) Comparison of size of the SAG with various treatments. Control embryos (n = 30), mif MOs (n = 14), mif MOs + RNA (n = 12), iclp MOs (n = 17), and iclp MOs + RNA (n = 15).

Expression Data

Genes:	alcama hmx3a
Antibody:	zn-5
Fish:	WT WT + MO1-cd74a + MO1-cd74b WT + MO1-ddt + MO2-ddt + MO4-mif
Condition:	chemical treatment: 4-iodo-6-phenylpyrimidine
Knockdown Reagents:	MO1-cd74a MO1-cd74b MO1-ddt MO2-ddt MO4-mif
Anatomical Terms:	cranial ganglion neuron projection otic vesicle statoacoustic (VIII) ganglion
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-cd74a + MO1-cd74b WT + MO1-ddt + MO2-ddt + MO4-mif
Knockdown Reagents:	MO1-cd74a MO1-cd74b MO1-ddt MO2-ddt MO4-mif
Observed In:	cranial ganglion cranial ganglion neuron projection dorsal anterior lateral line ganglion neuroblast statoacoustic (VIII) ganglion
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 363(1), Shen, Y.C., Thompson, D.L., Kuah, M.K., Wong, K.L., Wu, K.L., Linn, S.A., Jewett, E.M., Shu-Chien, A.C., and Barald, K.F., The cytokine macrophage migration inhibitory factor (MIF) acts as a neurotrophin in the developing inner ear of the zebrafish, Danio rerio, 84-94, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.