ZFIN Image: Schneider et al., 2010, Fig. 2

Image

Figure Caption

Fig. 2 Heart laterality defects after Nkd1 knockdown in DFCs. (A) RT-PCR using cDNA from wt or Nkd1MO-injected embryos: β-actin or Nkd1 were amplified. (B and C) Arrows denote efficient targeting of CtrlMO and Nkd1MO into the DFCs. (D–F) Bright field image of KV in wt (D), CtrlMO^DFC (E) and Nkd1MO^DFC(F) embryos, at 10-somite stage. Arrows indicate vesicle location; n = notochord. (G) Summary of KV morphology defects. (H–K) Arrows denote expression of nkx2.5 in the heart, on the left side in wt (H) and on the middle (I), right (J) and bilaterally (K) in Nkd1MO^DFC embryos. (L) Summary of cardiac jogging defects.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 348(1), Schneider, I., Schneider, P.N., Derry, S.W., Lin, S., Barton, L.J., Westfall, T., and Slusarski, D.C., Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning, 22-33, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.