FIGURE

Fig. 4

ID

ZDB-FIG-101011-17

Publication

Erickson et al., 2010 - Meis1 specifies positional information in the retina and tectum to organize the zebrafish visual system

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Fig. 4

Meis1 positively regulates smad1 expression in the developing eye. (A, B) mRNA in situ hybridization (ISH) for smad1 in 15-hpf wild-type and meis1 morphant (meis1MO) embryos. (C, D) Whole mount immunostains for phosphorylated Smad1/5/8 on 12-hpf wild-type and meis1 morphant embryos. (E-H) Phospho-Smad1/5/8 immunostains on 15-hpf embryos treated with meis1 morpholino (F), smad5 morpholino (G) or a combination of both morpholinos (H). (I-L) mRNA ISH for tbx5 in 15-hpf wild-type (I), meis1 morphant (J), smad5 morphant (K), and meis1-smad5 double morphant (L) embryos. Dotted lines outline the optic vesicle. All views are dorsal with anterior to the left.

Expression Data

Genes:	smad1 tbx5a
Antibody:	Ab1-smad
Fish:	AB AB + MO1-meis1b AB + MO3-smad5 AB + MO1-meis1b + MO3-smad5
Knockdown Reagents:	MO1-meis1b MO3-smad5
Anatomical Term:	optic vesicle
Stage Range:	5-9 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-meis1b AB + MO3-smad5 AB + MO1-meis1b + MO3-smad5
Knockdown Reagents:	MO1-meis1b MO3-smad5
Observed In:	dorsal/ventral pattern formation embryonic retina morphogenesis in camera-type eye
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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