FIGURE

Fig. 1

ID

ZDB-FIG-091125-13

Publication

Hippe et al., 2009 - The interaction of nucleoside diphosphate kinase B with Gbetagamma dimers controls heterotrimeric G protein function

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Fig. 1

NDPK A and B depletion in zebrafish embryos. Zebrafish embryos were injected with MO-Ctrl (4 ng), MO-NDPK A (8 ng) and MO-NDPK B (4 ng). (A) Immunoblot analysis of lysates prepared 72 hpf using specific antibodies against total fish NDPK (NDPK*), NDPK B, Gβ₁ and Gγ₂. β-actin served as loading and specificity control. (B) Relative NDPK activity (normalized to MO-Ctrl) was quantified as formation of ³H-GTP from ³H-GDP and ATP in lysates of embryos injected as indicated. Data are means ± SEM, n = 3, *, P < 0.05 vs. MO-Ctrl. (C) Co-immunoprecipitation of NDPK B and Gβ in zebrabrafish lysates. Endogenous Gβ or NDPK B was immunoprecipitated (IP) from zebrafish lysates. Association of NDPK B or Gβ was detected by immunoblotting (IB). Representative immunoblots are shown.

Expression Data

Genes:	gnb1a gng2 nme2b.1
Antibody:	Ab1-nme2
Fish:	WT WT + MO1-nme2b.2 WT + MO2-nme2b.1
Knockdown Reagents:	MO1-nme2b.2 MO2-nme2b.1
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-nme2b.2 WT + MO2-nme2b.1
Knockdown Reagents:	MO1-nme2b.2 MO2-nme2b.1
Observed In:	GTP biosynthetic process
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA