FIGURE

Fig. S3

ID

ZDB-FIG-101117-54

Publication

Hippe et al., 2009 - The interaction of nucleoside diphosphate kinase B with Gbetagamma dimers controls heterotrimeric G protein function

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Fig. S3

Rescue in NDPK B morphants. Zebrafish embryos were injected with MO-Ctrl (4 ng), NDPK B-mRNA (0.8 ng) and MO-NDPK B (4 ng) as well as co-injected with both MO-NDPK B (4 ng) and NDPK B-mRNA (0.8 ng) that lacks theMObinding site. (A) Representative immunoblots of zebrafish lysates prepared at 72 hpf confirmed mRNA driven NDPK B expression. (B) Quantitative analysis of rescued NDPK B morphants (4 ng) by co-injection of 0.8 ng NDPK B-mRNA according to phenotypic appearance. Data are means±SEM, n=3, 70–80 fishes respectively. Representive phenotypes ofWT(C),NDPKB morphants (D) and rescued embryos (E). (F) Rescue of cardiac contractility. Fractional shortening of the ventricular and atrial chamber of WT, NDPK B morphants and rescued morphants are shown. Data are means ± SEM, n = 10–15 for each condition, *, P < 0.05 vs. MO-NDPK B.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA