FIGURE

Fig. 9

ID

ZDB-FIG-090501-24

Publication

Esaki et al., 2009 - Mechanism of development of ionocytes rich in vacuolar-type H(+)-ATPase in the skin of zebrafish larvae

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Fig. 9

Lineage tracing showing a foxi3a-positive nature of NaK-MRC precursor. (A) Structures of the three expression vectors used for in vivo RFP labeling of atp1a1a.2-positive cells and in vivo GFP labeling of foxi3a-positive cells. (B–F) Demonstration that RFP is specifically expressed in the NaK-MRC subtype in the atp1a1a.2:RFP transgenic zebrafish line. In C, MitoTracker stains all types of MRCs. Blue arrows in panel D indicate MRCs other than NaK-MRC. (G–L) Examples of coexpression of RFP and GFP in the same cell under the influence of the promoters of the atp1a1a.2 and foxi3agenes in double transgenic zebrafish embryos. Dark areas in panels G–I represent spider-shaped melanocytes.

Expression Data

Genes:	atp1a1a.2 GFP RFP
Fish:	zf135Tg kca33Tg; zf135Tg; zf136Tg
Anatomical Term:	yolk integument
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 329(1), Esaki, M., Hoshijima, K., Nakamura, N., Munakata, K., Tanaka, M., Ookata, K., Asakawa, K., Kawakami, K., Wang, W., Weinberg, E.S., and Hirose, S., Mechanism of development of ionocytes rich in vacuolar-type H(+)-ATPase in the skin of zebrafish larvae, 116-129, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.