PUBLICATION

Mechanism of development of ionocytes rich in vacuolar-type H(+)-ATPase in the skin of zebrafish larvae

Authors
Esaki, M., Hoshijima, K., Nakamura, N., Munakata, K., Tanaka, M., Ookata, K., Asakawa, K., Kawakami, K., Wang, W., Weinberg, E.S., and Hirose, S.
ID
ZDB-PUB-090310-19
Date
2009
Source
Developmental Biology   329(1): 116-129 (Journal)
Registered Authors
Hoshijima, Kazuyuki, Kawakami, Koichi, Tanaka, Mikiko, Weinberg, Eric
Keywords
Mitochondria-rich cell, Foxi1, Foxi3a, Gcm2, Positive feedback loop, Zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Fluorescent Antibody Technique, Indirect
  • Immunohistochemistry
  • In Situ Hybridization
  • Keratinocytes/cytology
  • Keratinocytes/metabolism*
  • Larva/metabolism*
  • Microinjections
  • Mitochondria/metabolism*
  • Models, Biological
  • Oligonucleotides, Antisense/pharmacology
  • Proton-Translocating ATPases/metabolism*
  • Skin/cytology
  • Skin/metabolism*
  • Vacuoles/genetics
  • Vacuoles/metabolism*
  • Zebrafish/genetics
  • Zebrafish/metabolism
(all 19)
PubMed
19268451 Full text @ Dev. Biol.
Abstract
Mitochondrion-rich cells (MRCs), or ionocytes, play a central role in aquatic species, maintaining body fluid ionic homeostasis by actively taking up or excreting ions. Since their first description in 1932 in eel gills, extensive morphological and physiological analyses have yielded important insights into ionocyte structure and function, but understanding the developmental pathway specifying these cells remains an ongoing challenge. We previously succeeded in identifying a key transcription factor, Foxi3a, in zebrafish larvae by database mining. In the present study, we analyzed a zebrafish mutant, quadro (quo), deficient in foxi1 gene expression and found that foxi1 is essential for development of an MRC subpopulation rich in vacuolar-type H(+)-ATPase (vH-MRC). foxi1 acts upstream of Delta-Notch signaling that determines sporadic distribution of vH-MRC and regulates foxi3a expression. Through gain-and loss-of-function assays and cell transplantation experiments, we further clarified that (1) the expression level of foxi3a is maintained by a positive feedback loop between foxi3a and its downstream gene gcm2 and (2) Foxi3a functions cell-autonomously in the specification of vH-MRC. These observations provide a better understanding of the differentiation and distribution of the vH-MRC subtype.
Genes / Markers
Marker Marker Type Name
atp1a1a.2GENEATPase Na+/K+ transporting subunit alpha 1a, tandem duplicate 2
atp6v1abGENEATPase H+ transporting V1 subunit Ab
dlcGENEdeltaC
foxi1GENEforkhead box i1
foxi3aGENEforkhead box I3a
foxi3bGENEforkhead box I3b
gcm2GENEglial cells missing transcription factor 2
notch1aGENEnotch receptor 1a
tp63GENEtumor protein p63
1 - 9 of 9
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Figures
Figure Gallery (9 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-foxi3astandard conditionsPrim-5
WT + MO1-foxi3astandard conditionsPrim-5
WT + MO1-foxi3astandard conditionsLong-pec
WT + MO1-foxi3astandard conditionsLong-pec
WT + MO3-foxi1standard conditions5-9 somites
WT + MO3-foxi1standard conditions5-9 somites
WT + MO3-foxi1standard conditionsPrim-5
WT + MO3-foxi1standard conditionsPrim-5
WT + MO3-foxi1standard conditionsLong-pec
WT + MO5-gcm2standard conditionsPrim-5
1 - 10 of 18
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
kca33TgTransgenic Insertion
    m271
      		Unknown
      zf135TgTransgenic Insertion
        zf136TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          foxi1MO3-foxi1MRPHLNO
          foxi3aMO1-foxi3aMRPHLNO
          foxi3aMO2-foxi3aMRPHLNO
          foxi3bMO3-foxi3bMRPHLNO
          foxi3bMO4-foxi3bMRPHLNO
          gcm2MO5-gcm2MRPHLNO
          1 - 6 of 6
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          Fish
          Fish
          foxi1m271/m271
          kca33Tg; zf135Tg; zf136Tg
          kca33Tg; zf136Tg
          WT
          WT + MO1-foxi3a
          WT + MO3-foxi1
          WT + MO3-foxi3b
          WT + MO5-gcm2
          zf135Tg
          zf136Tg
          1 - 10 of 10
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          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          GAL4FFEFGGAL4FF
          GFPEFGGFP
          RFPEFGRFP
          1 - 3 of 3
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          Mapping
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          Citation
          Esaki, M., Hoshijima, K., Nakamura, N., Munakata, K., Tanaka, M., Ookata, K., Asakawa, K., Kawakami, K., Wang, W., Weinberg, E.S., and Hirose, S. (2009) Mechanism of development of ionocytes rich in vacuolar-type H(+)-ATPase in the skin of zebrafish larvae. Developmental Biology. 329(1):116-129.