FIGURE

Fig. 6

ID

ZDB-FIG-080715-7

Publication

Mei et al., 2008 - C1q-like inhibits p53-mediated apoptosis and controls normal hematopoiesis during zebrafish embryogenesis

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Fig. 6

Cell-cycle-progression defects in the C1q-like morphant embryos. (A) The up-regulated expression changes of p21^WAF1/CIP-1 (p21), p53, mdm2, bax and caspase 3 revealed by Q-RT-PCR in the C1q-like morphants. The ratio of these genes to β-actin in control embryos was set to 1, and all C1q-like MO treated embryos were normalized relative to this value. The date represent mean ± SD of three separate experiments. (B) The labeled G2/M phase cells of head regions in control (a), the C1q-like morphant (b) and co-knockdowns of C1q-like and p53 (c) embryos at 24 hpf recognized with the antibody against phosphorylated histone H3 (pH3). (C) A typical FACS analysis data of propidium-iodide-labeled cells in control (a), the C1q-like morphant (b) and co-knockdowns of C1q-like and p53 (c) embryos at 24 hpf. (D) The statistical data of three independent experiments of (C). *p < 0.05 and **p < 0.01.

Expression Data

Genes:	baxa casp3a cdkn1a mdm2 tp53
Antibody:	Ab1-h3
Fish:	WT WT + MO1-c1ql4l WT + MO1-c1ql4l + MO4-tp53
Knockdown Reagents:	MO1-c1ql4l MO4-tp53
Anatomical Terms:	cell whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-c1ql4l WT + MO1-c1ql4l + MO4-tp53
Knockdown Reagents:	MO1-c1ql4l MO4-tp53
Observed In:	cell cycle
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 319(2), Mei, J., Zhang, Q.Y., Li, Z., Lin, S., and Gui, J.F., C1q-like inhibits p53-mediated apoptosis and controls normal hematopoiesis during zebrafish embryogenesis, 273-284, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.