FIGURE

Fig. S4

ID

ZDB-FIG-070219-18

Publication

Hernandez et al., 2007 - Cyp26 enzymes generate the retinoic acid response pattern necessary for hindbrain development

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

cyp26 genes function redundantly to protect the anterior hindbrain from endogenous RA. cyp26a1 and cyp26c1 are responsible for setting the r3-r4 limit. (A,C,E,G) Knocking-down cyp26b1 and/or c1 has no effect on hindbrain patterning in wild-type embryos. (B) cyp26a1 mutants exhibit a mild expansion of r4. This effect is enhanced by knocking-down cyp26b1 (D), but hoxb1a expression is shifted to the presumptive cerebellum (En-expressing domains) by knocking-down cyp26c1 (F). Embryos depleted for all three cyp26 proteins exhibit a profound anterior shift of hoxb1 expression to the mid-hindbrain domain (H). Scale bar: 100 μm.

Expression Data

Genes:	en2b hoxb1a
Fish:	cyp26a1^rw716/rw716 + MO1-dnd1 cyp26a1^rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 cyp26a1^rw716/rw716 + MO1-cyp26b1 + MO1-dnd1 cyp26a1^rw716/rw716 + MO1-cyp26c1 + MO1-dnd1
Knockdown Reagents:	MO1-cyp26b1 MO1-cyp26c1 MO1-dnd1
Anatomical Terms:	midbrain midbrain hindbrain boundary rhombic lip rhombomere 4
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cyp26a1^rw716/rw716 + MO1-dnd1 cyp26a1^rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 cyp26a1^rw716/rw716 + MO1-cyp26b1 + MO1-dnd1 cyp26a1^rw716/rw716 + MO1-cyp26c1 + MO1-dnd1
Knockdown Reagents:	MO1-cyp26b1 MO1-cyp26c1 MO1-dnd1
Observed In:	hindbrain rhombomere 4
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development