- Title
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Chamber Specific Gene Expression Landscape of the Zebrafish Heart
- Authors
- Singh, A.R., Sivadas, A., Sabharwal, A., Vellarikal, S.K., Jayarajan, R., Verma, A., Kapoor, S., Joshi, A., Scaria, V., Sivasubbu, S.
- Source
- Full text @ PLoS One
ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. EXPRESSION / LABELING:
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Experimental validation of top ventricle-restricted putative novel protein coding genes.(A) Putative exon structure, human protein homologs and protein domain architecture of the candidate novel protein coding genes. (B) Validation of ventricle-restricted protein coding gene candidates using qRT-PCR. (C) Validation of ventricle-restricted protein coding gene candidates using WISH. XLOC_170573 (Novel 1), XLOC_159758 (Novel 2) and XLOC_215980 (Novel 3) show restricted expression in the ventricle. Scale bar: 100µm. A (Atrium); V (Ventricle) and BA (Bulbous arteriosus). EXPRESSION / LABELING:
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The zebrafish heart anatomy. (a) Cartoon of the adult zebrafish heart, (b) Dissected adult heart and the three chambers used for the present transcriptome analysis, (c) Atrium (d) Ventricle, (e) Bulbus arteriosus. Scale bar: 500µm. |
Experimental validation of chamber-restricted differentially expressing protein coding genes. Ventricle-restricted genes (a) irx1a and (b) ldb3a, Atrium- restricted gene (c) vtg2, Bulbus arteriosus-restricted gene (d) rgs5a. (A) Atrium, (V) Ventricle and (BA) Bulbus arteriosus. Scale bar: 100 µm. See text for detailed information. EXPRESSION / LABELING:
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