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Fig. 6
Experimental validation of top ventricle-restricted putative novel protein coding genes.(A) Putative exon structure, human protein homologs and protein domain architecture of the candidate novel protein coding genes. (B) Validation of ventricle-restricted protein coding gene candidates using qRT-PCR. (C) Validation of ventricle-restricted protein coding gene candidates using WISH. XLOC_170573 (Novel 1), XLOC_159758 (Novel 2) and XLOC_215980 (Novel 3) show restricted expression in the ventricle. Scale bar: 100µm. A (Atrium); V (Ventricle) and BA (Bulbous arteriosus).
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