PUBLICATION

Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium

Authors
Mytlis, A., Kumar, V., Qiu, T., Deis, R., Hart, N., Levy, K., Masek, M., Shawahny, A., Ahmad, A., Eitan, H., Nather, F., Adar-Levor, S., Birnbaum, R.Y., Elia, N., Bachmann-Gagescu, R., Roy, S., Elkouby, Y.M.
ID
ZDB-PUB-220514-11
Date
2022
Source
Science (New York, N.Y.)   376(6599): eabh3104 (Journal)
Registered Authors
Bachmann-Gagescu, Ruxandra, Birnbaum, Ramon, Elkouby, Yaniv M., Masek, Markus, Roy, Sudipto
Keywords
none
MeSH Terms
  • Animals
  • Centromere/genetics
  • Centromere/physiology
  • Chromosome Pairing*/genetics
  • Chromosome Pairing*/physiology
  • Cilia*/physiology
  • Female
  • Fertility/physiology
  • Mice
  • Morphogenesis
  • Oocytes*/growth & development
  • Oogenesis*/genetics
  • Oogenesis*/physiology
  • Ovary*/growth & development
  • Telomere/genetics
  • Telomere/physiology
  • Zebrafish/genetics
  • Zebrafish/physiology
(all 18)
PubMed
35549308 Full text @ Science
Abstract
A hallmark of meiosis is chromosomal pairing, which requires telomere tethering and rotation on the nuclear envelope via microtubules, driving chromosome homology searches. Telomere pulling toward the centrosome forms the "zygotene chromosomal bouquet". Here, we identified the "zygotene cilium" in oocytes. This cilium provides a cable system for the bouquet machinery, extending throughout the germline cyst. Using zebrafish mutants and live manipulations, we demonstrate that the cilium anchors the centrosome to counterbalance telomere pulling. The cilium is essential for bouquet and synaptonemal complex formation, oogenesis, ovarian development, and fertility. Thus, a cilium represents a conserved player in zebrafish and mouse meiosis, which sheds light on reproductive aspects in ciliopathies, and suggests that cilia can control chromosomal dynamics.
Genes / Markers
Marker Marker Type Name
actb2GENEactin, beta 2
armc9GENEarmadillo repeat containing 9
bcl2aGENEBCL2 apoptosis regulator a
cc2d2aGENEcoiled-coil and C2 domain containing 2A
cep290GENEcentrosomal protein 290
cep55lGENEcentrosomal protein 55 like
cetn4GENEcentrin 4
foxj1bGENEforkhead box J1b
kif7GENEkinesin family member 7
tp53GENEtumor protein p53
1 - 10 of 10
Show
Figures
Figure Gallery (6 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
bgu100TgTransgenic Insertion
    cu6TgTransgenic Insertion
      fh297
        		Point Mutation
        hsc5TgTransgenic Insertion
          i271
            		Small Deletion
            kca66TgTransgenic Insertion
              tsu10GtTransgenic Insertion
              vcc41TgTransgenic Insertion
                w38
                  		Point Mutation
                  zdf1
                    		Point Mutation
                    1 - 10 of 11
                    Show
                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    No data available
                    Fish
                    No data available
                    Antibodies
                    Orthology
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    EGFPEFGEGFP
                    GFPEFGGFP
                    mCherryEFGmCherry
                    mRFP1EFGmRFP1
                    1 - 4 of 4
                    Show
                    Mapping
                    Your Input Welcome
                    We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
                    Citation
                    Mytlis, A., Kumar, V., Qiu, T., Deis, R., Hart, N., Levy, K., Masek, M., Shawahny, A., Ahmad, A., Eitan, H., Nather, F., Adar-Levor, S., Birnbaum, R.Y., Elia, N., Bachmann-Gagescu, R., Roy, S., Elkouby, Y.M. (2022) Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium. Science (New York, N.Y.). 376(6599):eabh3104.