Fig. 6

Yap modulates atrial cardiomyocyte elongation.a Schematic of mosaic Yap1DN overexpression in CMs. b, c 3D confocal images of 144 hpf atria from control mosaic labeled (b) (creERT2-, TagBFP+) and Yap1DN mosaic overexpressing (c) (creERT2+, mCherry+) larvae; CM membranes shown in cyan (myl7:EGFP-Hsa.HRAS), control mosaic labeling in magenta (hspl70:LSL-NLSyap1DN, myh6:creERT2-), and Yap1DN mosaic labeling in yellow (hspl70:LSL-NLSyap1DN, myh6:creERT2+). d Percentage of elongating CMs from control labeled or Yap1DN overexpressing CMs (n = 8 hearts, 15 control labeled CMs; n = 8 hearts, 27 YAP1DN overexpressing CMs). e schematic of Yap1DN overexpression using the stable transgenic line. f Percentage of left, centered, and right oriented hearts at 30 hpf from uninjected, water injected, and cre mRNA injected hspl70:LSL-NLSyap1DN embryos (n = 10 uninjected controls, n = 24 water injected controls, and n = 23 cre mRNA injected embryos). g, h 3D airyscan images of 144 hpf atria from hspl70:LSL-NLSyap1DN control larvae (g) and hspl70:LSL-NLSyap1DN; myh6:CreERT2 siblings (h); CM membranes shown in cyan (myl7:EGFP-Hsa.HRAS); larvae were heat shocked once daily from 48 hpf and kept in Tamoxifen from 60 to 144 hpf, refreshed once daily. i, Percentage of elongating atrial CMs at 144 from hspl70:LSL-NLSyap1DN control larvae and hspl70:LSL-NLSyap1DN; myh6:CreERT2 siblings (n = 9 Cre- controls and n = 14 Cre+ siblings, two-tailed Mann-Whitney test). Error bars are mean ± SD.