PUBLICATION
Distinct mechanisms regulate ventricular and atrial chamber wall formation
- Authors
- Albu, M., Affolter, E., Gentile, A., Xu, Y., Kikhi, K., Howard, S., Kuenne, C., Priya, R., Gunawan, F., Stainier, D.Y.R.
- ID
- ZDB-PUB-240918-22
- Date
- 2024
- Source
- Nature communications 15: 81598159 (Journal)
- Registered Authors
- Albu, Marga, Priya, Rashmi, Stainier, Didier
- Keywords
- none
- Datasets
- GEO:GSE249149
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Shape
- Gene Expression Regulation, Developmental
- Heart Atria*/cytology
- Heart Atria*/metabolism
- Heart Ventricles*/cytology
- Heart Ventricles*/metabolism
- Morphogenesis
- Myocytes, Cardiac*/cytology
- Myocytes, Cardiac*/metabolism
- Receptors, Notch/metabolism
- Signal Transduction
- Zebrafish*
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 39289341 Full text @ Nat. Commun.
Citation
Albu, M., Affolter, E., Gentile, A., Xu, Y., Kikhi, K., Howard, S., Kuenne, C., Priya, R., Gunawan, F., Stainier, D.Y.R. (2024) Distinct mechanisms regulate ventricular and atrial chamber wall formation. Nature communications. 15:81598159.
Abstract
Tissues undergo distinct morphogenetic processes to achieve similarly shaped structures. In the heart, cardiomyocytes in both the ventricle and atrium build internal structures for efficient contraction. Ventricular wall formation (trabeculation) is initiated by cardiomyocyte delamination. How cardiomyocytes build the atrial wall is poorly understood. Using longitudinal imaging in zebrafish, we found that at least 25% of the atrial cardiomyocytes elongate along the long axis of the heart. These cell shape changes result in cell intercalation and convergent thickening, leading to the formation of the internal muscle network. We tested factors important for ventricular trabeculation including Nrg/ErbB and Notch signaling and found no evidence for their role in atrial muscle network formation. Instead, our data suggest that atrial cardiomyocyte elongation is regulated by Yap, which has not been implicated in trabeculation. Altogether, these data indicate that distinct cellular and molecular mechanisms build the internal muscle structures in the atrium and ventricle.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping