FIGURE

Fig. 2.

ID

ZDB-FIG-240527-64

Publication

Rutherford et al., 2024 - Macrophage transplantation rescues RNASET2-deficient leukodystrophy by replacing deficient microglia in a zebrafish model

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Fig. 2.

Macrophage transplantation reduces early neuropathology in rnaset2 mutants. (A and B) Quantification of 4C4-expression of transplant-derived cells in microglia-depleted versus nondepleted rnaset2 hosts at 5 (A) and 8 dpf (B) reveals reduced expression of 4C4 by transplanted cells in nondepleted hosts. Mann Whitney test. Three biological replicates, n = 29 to 37 (A), three biological replicates, n = 23 to 32 (B). (C and D) Macrophage transplantation reduces the number of uncleared apoptotic cells in the optic tectum of 5 (C) and 8 dpf (D) rnaset2 mutants. Kruskal–Wallis test with Dunn’s multiple comparisons. Four biological replicates, n = 25 to 38 (C), three biological replicates, n = 25 to 48 (D). (E and F) qPCR reveals that isg15 (E) and mxa (F) expression is reduced in transplanted animals, relative to WT controls. Kruskal–Wallis test with Dunn’s multiple comparisons. Three biological replicates, 15 embryos pooled per replicate.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA