ZFIN Image: Rutherford et al., 2024, Fig. 2.

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Fig. 2.

Macrophage transplantation reduces early neuropathology in rnaset2 mutants. (A and B) Quantification of 4C4-expression of transplant-derived cells in microglia-depleted versus nondepleted rnaset2 hosts at 5 (A) and 8 dpf (B) reveals reduced expression of 4C4 by transplanted cells in nondepleted hosts. Mann Whitney test. Three biological replicates, n = 29 to 37 (A), three biological replicates, n = 23 to 32 (B). (C and D) Macrophage transplantation reduces the number of uncleared apoptotic cells in the optic tectum of 5 (C) and 8 dpf (D) rnaset2 mutants. Kruskal–Wallis test with Dunn’s multiple comparisons. Four biological replicates, n = 25 to 38 (C), three biological replicates, n = 25 to 48 (D). (E and F) qPCR reveals that isg15 (E) and mxa (F) expression is reduced in transplanted animals, relative to WT controls. Kruskal–Wallis test with Dunn’s multiple comparisons. Three biological replicates, 15 embryos pooled per replicate.

Acknowledgments

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