Transplantation rescues rnaset2 mutant swimming behavior and survival. (A) Live tracking of larval swimming behavior reveals that transplanted rnaset2 mutants swim greater distances than nontransplanted controls. Kruskal–Wallis test with Dunn’s multiple comparisons. Three biological replicates, n = 52 to 60. (B) Representative traces of free-swimming behavior of zebrafish larvae. The green line represents slow movements (3.0 to 6.6 mm/s), red line represents fast movements (>6.6 mm/s). (C) Tracking of 4 wpf juvenile behavior reveals that transplantation restores rnaset2 swimming behavior to WT levels. Kruskal–Wallis test with Dunn’s multiple comparisons, n = 11 to 31. (D) Swimming behavior was comparable between rnaset2 microglia-depleted transplanted animals that had persistent engraftment of transplanted cells and those which did not. Mann–Whitney U test, n = 12 to 19. (E and F) Percentage survival of transplanted rnaset2 mutants is greater than microglia-depleted sham controls at 4 wpf. Pairwise comparisons and corresponding P values shown in F, bold indicates significance. Log rank Mantel–Cox test with Bonferroni’s multiple comparison correction (Bonferroni-corrected threshold: P < 0.00833; family-wise significance threshold: P < 0.05). Three biological replicates, n = 60 to 86.
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