FIGURE

Fig. 3.

ID

ZDB-FIG-230420-80

Publication

Snodgrass et al., 2023 - Therapeutic targeting of vascular malformation in a zebrafish model of hereditary haemorrhagic telangiectasia

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Fig. 3.

Vegf inhibition between 2 and 3 dpf rescues the abnormal trunk and cerebral vessel phenotypes of eng^mu130 mutants. (A) Experimental plan for rescuing the eng mutant phenotype in zebrafish using AV951. dpf, days post fertilisation. (B) Representative maximum-intensity projection of the trunk vasculature of eng^mu130 and WT embryos±AV951 (25 nM) treatment for 24 h. DA, dorsal aorta; PCV, posterior cardinal vein. Scale bar: 150 μm. (C) Representative maximum-intensity projection of the cerebral vasculature of eng^mu130 and WT embryos±AV951 (25 nM) treatment for 24 h. Scale bar: 100 μm. (D) DA diameter in eng^mu130 and WT embryos±AV951 treatment. (E) PCV diameter in eng^mu130 and WT embryos±AV951 treatment. (F) Intersegmental blood vessel (ISV) diameters in eng^mu130 and WT embryos±AV951 treatment. (G) Percentage of open ISVs in eng^mu130 and WT embryos±AV951 treatment. (H) Number of kugeln in eng^mu130 and WT embryos±AV951 treatment. (I) BA diameter in eng^mu130 and WT embryos±25 nM AV951 treatment. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns, not significant (two-way ANOVA with Tukey post-hoc test, 10 animals/group).

Expression Data

Gene:	mCherry
Fish:	s916Tg eng^mu130/mu130; s916Tg
Condition:	chemical treatment by environment: tivozanib
Anatomical Terms:	brain vasculature plasma membrane dorsal aorta plasma membrane intersegmental vessel plasma membrane posterior cardinal vein plasma membrane
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s916Tg eng^mu130/mu130; s916Tg
Condition:	chemical treatment by environment: tivozanib
Observed In:	basilar artery blood vessel endothelial cell plasma membrane bounded cell projection brain vasculature blood vessel endothelial cell dorsal aorta intersegmental vessel posterior cardinal vein
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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