FIGURE

Fig. 6

ID

ZDB-FIG-230405-13

Publication

Tan et al., 2022 - Pax2a, Sp5a and Sp5l act downstream of Fgf and Wnt to coordinate sensory-neural patterning in the inner ear

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Fig. 6

Effects of signal modulation on reporters of Fgf and Wnt signaling. (A) Dorsolateral views showing expression of Wnt reporter top:gfp (a–e) or Fgf-target etv5b (f–j) at 24 hpf under conditions indicated at the top. (B) Cross-sections (dorsal to the top, medial to the left) of etv5b-stained embryos passing through the anterior or middle the otic vesicle, as indicated on the left. Black arrows (e, f) indicate regions of weaker expression of etv5b. (C) Dorsolateral views showing expression of fgf3, fgf8a and fgf10a at 24 hpf in control and BIO-treated embryos. Pharyngeal endoderm (pe) is marked (Ca, Cb). (D) Cross-sections of embryos showing expression of sp5a and top:gfp at 24 hpf following treatments from 12 hpf, as indicated at the top. Scale bars (Aa, Ba, Ca, Da), 50 μm.

Expression Data

Genes:	etv5b fgf3 fgf8a fgf10a GFP sp5a
Fish:	w25Tg/w25Tg AB w32Tg/w32Tg x17Tg/x17Tg w25Tg/w25Tg; w32Tg/w32Tg w25Tg/w25Tg; x17Tg/x17Tg
Conditions:	chemical treatment by environment: 6-bromoindirubin-3'-oxime chemical treatment by environment: IWR-1-endo chemical treatment by environment: SU5402
Anatomical Terms:	otic vesicle otic vesicle medial region pharyngeal endoderm
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	w25Tg/w25Tg AB x17Tg/x17Tg w25Tg/w25Tg; w32Tg/w32Tg
Conditions:	chemical treatment by environment: 6-bromoindirubin-3'-oxime chemical treatment by environment: IWR-1-endo chemical treatment by environment: SU5402
Observed In:	otic vesicle otic vesicle medial region pharyngeal endoderm
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 492, Tan, A.L., Mohanty, S., Guo, J., Lekven, A.C., Riley, B.B., Pax2a, Sp5a and Sp5l act downstream of Fgf and Wnt to coordinate sensory-neural patterning in the inner ear, 139-153, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.